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SPY620-actin

SPY620-actin

SPY620-actin is a bright fluorogenic, non toxic, cell permeable and highly specific live cell probe for fluorescence imaging of actin.

Product contains 1 vial of SPY620-actin in lyophilised form allowing to prepare 50 ul of 1000x DMSO stock solution (100 stainings*).

产品参数
  • 品牌 Spirochrome
    品名 SPY620-actin
    货号 SC402
    规格 100 stainings
    售价 询价
    λabs 619 nm
    λfl 636 nm
    lifetime 2.9 ns
    推荐的STED损耗激光波长 775 nm
    运输 常温
    储存 -20℃
    能否用于固定细胞 可用于PFA固定的细胞
产品描述

SPY620-actin is a bright red & non toxic live and fixed cells F-actin stain based on our SPY™ dyes series. Its optimized structure allows quick labeling of F-actin in live & fixed cells with high specificity and very low background. SPY620-actin stains F-actin of live or fixed cells without the need for genetic manipulation or overexpression of fluorescent proteins. Its emission in the red minimizes phototoxicity and sample autofluorescence. SPY620-actin enables multicolor imaging with SPY505,SPY555, SPY650, SPY700,SiR&GFP.  SPY620-actin can be imaged with e.g. using 610/20 nm and 650/40 nm excitation and emission filters respectively. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. Probe quantity allows 100 staining experiments.*

 

*Based on the following conditions: 0.5 ml staining solution / staining experiment with 1x probe concentration. The number of staining experiments can be further increased by reducing the staining solution volume or the probe concentration.