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SPY505-CA

SPY505-CA

SPY505-CA (also known as MaP510-Halo) is a bright, fluorogenic, cell permeable and green emitting ChloroAlkane (CA) substrate for Halo-Tag labeling.

Product contains 1 vial of SPY505-CA (10 nmol) in lyophilised form allowing to prepare 50 ul of 1000x DMSO stock solution (100 stainings*).

产品参数
  • 品牌 Spirochrome
    品名 SPY505-CA
    货号 SC106
    规格 100 stainings
    售价 询价
    λabs 512 nm
    λfl 531 nm
    λSTED 595 nm
    荧光寿命  4.0 ns
    运输 常温
    储存 -20℃
    能否用于固定细胞 可用甲醇固定
 
 
产品描述

SPY505-CA is the ChloroAlkane (CA) derivative of our SPY505 fluorophore. It emits light in the green part of the UV-ViS spectrum, it is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. SPY505-CA can be imaged with standard FITC or YFP settings. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. It has the same structure as MaP510-halo, the name used in its original publication in Nature Chemistry1).

ChloroAlkane (CA) is the substrate of the self labeling tag Halo-Tag. Upon reaction with a CA derivative, Halo-Tag forms a covalent bond with the substrate. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as Halo-Tag fusion.

*Based on the following conditions: 0.5 ml staining solution / staining experiment with 1x probe concentration. The number of staining experiments can be further increased by reducing the staining solution volume or the probe concentration.

产品说明书：SPY505-CA datasheet

产品SDS下载：SPY505-CA SDS

产品光谱：Ex/Em spectra (excel format)

 

 

参考文献：1. “A general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy”; Lu Wang, et. al.; Nature Chemistry 12, 165-172, 2020.