SPY555-CA

产品描述

SPY555-CA is the ChloroAlkane (CA) derivative of our SPY555 fluorophore. It emits light in the orange part of the UV-ViS spectrum, it is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. SPY555-CA can be imaged with a standard Cy3 filterset. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. It has the same structure as MaP555-halo or MaP555-CA, the name used in its original publication in Nature Chemistry1).

ChloroAlkane (CA) is the substrate of the self labeling tag Halo-Tag. Upon reaction with a CA derivative, Halo-Tag forms a covalent bond with the substrate. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as Halo-Tag fusion.

*Based on the following conditions: 0.5 ml staining solution / staining experiment with 1x probe concentration. The number of staining experiments can be further increased by reducing the staining solution volume or the probe concentration.

产品说明书：SPY555-CA datasheet

产品SDS下载： SPY555-CA SDS

产品光谱： Ex/Em spectra (excel format)

参考文献：1. “A general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy”; Lu Wang, et. al.; Nature Chemistry 12, 165-172, 2020.

品牌	Spirochrome
品名	SPY555-CA
货号	SC206
规格	100 stainings
售价	询价
λ_abs	555 nm
λ_fl	580 nm
λ_STED	660 or 775 nm
荧光寿命	2.3 ns
运输	常温
储存	-20℃
能否用于固定细胞	可用甲醇固定