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SiR-CA

SiR-CA

SiR-CA is a bright, fluorogenic, cell permeable and far red emitting ChloroAlkane (CA) substrate for Halo-Tag labeling.

Product contains 1 vial of SiR-CA (10 nmol) in lyophilised form allowing to prepare 50 ul of 1000x DMSO stock solution (100 stainings*).

产品参数
  • 品牌 Spirochrome
    品名 SiR-CA
    货号 SC506
    规格 100 stainings
    售价 询价
    λabs 652 nm
    λfl 674 nm
    λSTED 775 nm
    荧光寿命  3.2 ns
    运输 常温
    储存 -20℃
    能否用于固定细胞 可用甲醇固定
 
 
产品描述

SiR-CA is the ChloroAlkane (CA) derivative of our Silicon Rhodamine (SiR) fluorophore. It emits light in the far red part of the UV-ViS spectrum, it is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. SiR-CA can be imaged with a standard Cy5 filterset. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. It has the same structure as SiR-halo, the name used in its original publication in Nature Chemistry1).

ChloroAlkane (CA) is the substrate of the self labeling tag Halo-Tag. Upon reaction with a CA derivative, Halo-Tag forms a covalent bond with the substrate. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as Halo-Tag fusion.

*Based on the following conditions: 0.5 ml staining solution / staining experiment with 1x probe concentration. The number of staining experiments can be further increased by reducing the staining solution volume or the probe concentration.

产品说明书：SiR-CA datasheet

产品SDS下载： SiR-CA SDS

产品光谱：Ex/Em spectra (excel format)

 

 

参考文献：1. “A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins”; G. Lukinavicius, et. al.; Nature Chemistry 5, 132–139 (2013).