SPY700-CA is the ChloroAlkane (CA) derivative of our SPY700 fluorophore. It emits light in the far red part of the UV-ViS spectrum, it is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. SPY700-CA can be imaged with Cy5 filtersets but using a 700 nm LP emission filter is best. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. It has the same structure as MaP700-halo, the name used in its original publication in Nature Chemistry1).
ChloroAlkane (CA) is the substrate of the self labeling tag Halo-Tag. Upon reaction with a CA derivative, Halo-Tag forms a covalent bond with the substrate. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as Halo-Tag fusion.
